AIM: The goal of the study was to compare the kinetic responses of heart rate (HR) and pulmonary (VO2pulm) and muscular (VO2musc) oxygen uptake during dynamic leg exercise across different acute ambient temperature conditions in a climatic chamber. METHODS: Thirteen physically healthy, active, male volunteers demonstrated pseudorandom binary sequence (PRBS) work rate (WR) changes between 30 and 80 W at 15 degrees C, 25 degrees C and 35 degrees C, respectively. HR was measured beat-to-beat using an echocardiogram and VO2pulm by breath-by-breath gas exchange; VO2musc estimations were assessed by applying a circulatory model and cross-correlation functions. RESULTS: No significant differences were observed across the various temperature conditions in each case for HR, VO2pulm or VO2musc kinetics (p > 0.05). Baroreflex regulation based on HR kinetics does not seem to be influenced between ambient temperatures of 15 degrees C and 35 degrees C during dynamic exercise. CONCLUSIONS: The results imply that ambient temperatures of 15 degrees C, 25 degrees C and 35 degrees C have no effect on HR, VO2pulm or VO2musc kinetics during dynamic moderate exercise. The applied approach may be of interest for assessments of the cardio-pulmonary and respiratory health statuses of individuals working or performing sports in extreme temperature environments. Furthermore, differentiation between systemic (e.g. cardio-dynamic: HR) and specific (e.g. exercising tissues: VO2musc) determinants of the relevant physiological systems may improve the evaluation of an individual's health status.