The Operational Framework is intended as a guide for one-health operations, from project and program scoping and identification stages to design and implementation, including monitoring and evaluation, to help optimize investments. Examples are provided in each section to assist sectors in identifying relevant points for participation; each sector will likely identify additional relevance and ideas for operationalizing one-health in reviewing the examples, as well as in the course of developing one-health programs (or in attempting to integrate one-health into existing programs). It opens the door for genuine collaboration and shared gains to address pressing issues central to the World Bank’s focus — noting that public health systems will only be stronger by integrating humans, animals and the environment.