This study was investigated the density and monthly distribution of indoor and outdoor microfungi in six different residential houses in Tekirdag City through the exposure of Petri dishes containing Rose-Bengal Streptomycin Agar media. Samples were collected in 1-month intervals over a period of 12 months between March, 2001, and February, 2002. We used 432 Petri dishes and counted a total of 4,205 microfungi colonies, 1,790 from indoor air and 2,415 from outdoor air. As a result, 42 species belonging to 12 genera were identified. The most frequent fungal genera were Penicillium (28.61%), Cladosporium (16.08%) and Alternaria (15.98%). While Penicillium (40.61%) and Cladosporium (15.92%) were the dominant genera of indoor air, Alternaria (20.62%) and Penicillium (19.71%) were isolated most frequently from outdoor air (Table 3). Alternaria citri (10.15%) and Penicillium brevicompactum (10.15%) were found to be the most frequent among the 42 identified species. While P. brevicompactum (19.55%) and Aspergillus niger (6.37%) were the most frequent indoor species, A. citri (13.37%) and Cladosporium cladosporioides (8.20%) were the most frequent outdoor species. Linear Regression Analysis was applied to determine whether or not there was a relationship between the number of colonies of isolated fungal genera and meteorological factors during the research period. Correlations between the presence of Aspergillus and temperature, relative humidity, duration of sunny periods and agents of air pollution such as SO2 and PM were statistically significant. No significant correlations, however, were found between other fungal genera and environmental variables. © Springer Science+Business Media B.V. 2008.