Pollen data recorded by a single sampler in any given city often fail to reflect particular events occurring in surrounding areas. This is frequently overlooked when interpreting aerobiological results, and therefore pollen data obtained in urban areas may not necessarily be representative of the situation in more rural areas of the same city. Our purpose is to assess differences between allergenic pollen concentrations recorded in an urban area (Eskulap) and a rural/suburban (Morasko) of the same city from 2005 to 2007. Anova-Manova Scheffe, Spearman correlation and Mixed-design ANOVA whitin-subjects effects tests were applied. The results obtained have showed longer pollen seasons, earlier starts of flowering and later conclusion in the urban area. The participation of arboreal pollen in the pollen fall of both sites had enough significative similarities to determine that is linked to regional conditions, while differences in the atmospheric pollen content of herbaceous plants between sites should be due to local conditions.