OBJECTIVE: Most evidence comes from studies show that ambient ozone(O(3)) pollution has become a big issue in China. Few studies have investigated the impact of ozone spatiotemporal patterns on respiratory mortality and cardiovascular mortality in Nanchang city. Thus, this study aimed to explore the health effect of ozone exposure on respiratory mortality and cardiovascular mortality in Nanchang, Jiangxi Province. METHODS: Using the daily mortality data, atmospheric routine monitoring data and meteorological data in Nanchang from 2014 to 2020, we performed a generalized additive model (GAM) based on the poisson distribution in which time-series analysis to calculate the risk correlation between respiratory mortality and cardiovascular mortality and ozone exposure level (8h average ozone concentration, O(3)-8h). Besides, analyses were also stratified by season, age and sex. RESULTS: In the single-pollutant model, for every 10 μg/m(3) increase in ozone, respiratory mortality increased 1.04% with 95% confidence interval (CI) between 0.04 and 1.68%, and cardiovascular mortality increased 1.26% (95%CI: 0.68 ~ 1.83%). In the multi-day moving average lag model, the mortality of respiratory diseases and cardiovascular diseases reached a relative risk peak on the cumulative lag5 (1.77%,95%CI: 0.99 ~ 2.57%) and the cumulative lag3 (1.68%,95%CI: 0.93 ~ 2.45%), respectively. The differences were statistically significant (P < 0.05). Results of the stratified analyses showed the effect value of respiratory mortality in people aged ≥65 years was higher than aged <65 years, whereas the greatest effect of cardiovascular mortality in people aged <65 years than aged ≥65 years. Ozone had a more profound impact on females than males in respiratory diseases and cardiovascular diseases. In winter and spring, ozone had a obvious impact on respiratory mortality, and effects of ozone pollution on cardiovascular mortality were stronger in summer and winter. There was a statistically significant difference of respiratory mortality in winter and spring and of cardiovascular mortality in summer and winter (P < 0.05). CONCLUSIONS: In the long run, the more extreme the pollution of ozone exposure, the higher the health risk of residents' respiratory mortality and cardiovascular mortality. Therefore, the government should play an important role in the prevention and control ways of decreasing and eliminating the ozone pollution to protect the resident's health. The findings provide valuable data for further scientific research and improving environmental policies in Nanchang city.