It has been reported previously that people who are acclimated to naturally ventilated (NV) environments respond to hot and warm environments differently than people who are acclimated to air-conditioned (AC) environments. However, it is not clear whether physiological acclimatization contributes to this discrepancy. To study whether living and working in NV or AC environments for long periods of time can lead to different types of physiological acclimatization, and whether physiological acclimatization has an important influence on people's responses of thermal comfort, measurements of physiological reactions (including skin temperature, sweat rate, heart rate variability, and heat stress protein 70) and thermal comfort responses were conducted in a 'heat shock' environment (climate chamber) with 20 people (10 in the NV group and 10 in the AC group). The results showed that the NV group had a significantly stronger capacity for physiological regulation to the heat shock than the AC group. In other words, the NV group did not feel as hot and uncomfortable as the AC group did. These results strongly indicate that living and working in indoor thermal environments for long periods of time affects people's physiological acclimatization. Also, it appears that long-term exposure to stable AC environments may weaken people's thermal adaptability. PRACTICAL IMPLICATIONS: This study examined the psychological and physiological differences of thermal adaptability of people used to air-conditioned environments and naturally ventilated environments. The results suggested that long-term exposure to stable air-conditioned environments may weaken people's thermal adaptability. Therefore, it might be advantageous for people to spend less time in static air-conditioned environments; this is not only because of its possible deleterious impact on people's physiological adaptability, but also because the air-conditioners' high-energy consumption will contribute to the effects of global warming.