Power grid outdoor workers are usually exposed to hot environments and could suffer the threats to occupational health and safety like heat strain and injury. In order to predict and assess the thermophysiological responses of grid workers in the heat, the clothing thermal insulation of grid worker ensembles was measured by a thermal manikin and a multi-segment human bioheat model was employed to evaluate the thermophysiological response parameters of grid workers such as core temperature, skin temperature and sweat loss. The results show that working in a hot environment can cause a obvious increase in core temperature and skin temperature of grid workers, and the acceptable maximum working time of grid workers varies greatly in different hot environments. A reasonable work organization strategy can effectively decrease the core temperature and sweat loss, increasing the duration of acceptable maximum working time for grid workers. This study is helpful to assess heat-related risks of grid workers and support power grid companies to rationalize work organization strategies and personal protection guidelines.