AIM: Studies fall short when it comes to determining the relationship between thermal comfort and cardiovascular diseases. Studies examining the relationship between thermal comfort conditions and human health in Turkey, located in the transition zone of air masses at mid-latitudes, are quite limited. This is the first study conducted in Turkey that deals with thermal comfort conditions and CVDs, which is the leading cause of death. This study aimed to examine the relationship between thermal comfort conditions and CVDs of Amasya, a medium-sized exemplary Turkish city. SUBJECT AND METHODS: To determine the thermal comfort conditions in the study area between 2014-2019, the physiologically equivalent temperature (PET) index obtained from the Rayman model, which uses hourly air temperature (ºC), relative humidity (%), wind speed (m/s), and cloud cover (octa) data, was used. The relationship between PET values and CVDs was determined by Pearson correlation analysis and linear regression analysis. RESULTS: The study indicated a negative, high, and moderate correlation between PET values and cardiovascular diseases (p < 0.001). The results show that when PET values increase by 1 ºC, patient admissions will decrease by about 104 to 108 patients (-104.737 to -108.619 units.). CONCLUSION: These results can be informative and guiding for both the protection of public health and studies on climate change and human health.