It is important to quantify human heat exposure in order to evaluate and mitigate the negative impacts of heat on human well-being in the context of global warming. This study proposed a human-centric framework to examine human personal heat exposure based on anonymous GPS trajectories data mining and urban microclimate modeling. The mean radiant temperature (T-mrt) that represents human body's energy balance was used to indicate human heat exposure. The meteorological data and high-resolution 3D urban model generated from multispectral remotely sensed images and LiDAR data were used as inputs in urban microclimate modeling to map the spatio-temporal distribution of the T-mrt, in the Boston metropolitan area. The anonymous human GPS trajectory data collected from fitness Apps was used to map the spatio-temporal distribution of human outdoor activities. By overlaying the anonymous GPS trajectories on the generated spatio-temporal maps of T-mrt, this study further examined the heat exposure of runners in different age-gender groups in the Boston area. Results show that there is no significant difference in terms of heat exposure for female and male runners. The female runners in the age of 45-54 are exposed to more heat than female runners of 18-24 and 25-34, while there is no significant difference among male runners. This study proposed a novel method to estimate human heat exposure, which would shed new light on mitigating the negative impacts of heat on human health.